pAAV-hSyn-BRIPO
(Plasmid
#215551)
-
PurposeAAV transfer plasmid for BRIPO (Bioluminescent Red Indicator for Potassium)
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 215551 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepAAV-hSyn
-
Vector typeAAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameBRIPO
-
SpeciesSynthetic
- Promoter hSyn promotor
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ttcccggccaccttggtc (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV-hSyn-BRIPO was a gift from Huiwang Ai (Addgene plasmid # 215551 ; http://n2t.net/addgene:215551 ; RRID:Addgene_215551) -
For your References section:
Bioluminescence Imaging of Potassium Ion Using a Sensory Luciferin and an Engineered Luciferase. Zhao S, Xiong Y, Sunnapu R, Zhang Y, Tian X, Ai HW. J Am Chem Soc. 2024 May 15;146(19):13406-13416. doi: 10.1021/jacs.4c02473. Epub 2024 May 2. 10.1021/jacs.4c02473 PubMed 38698549
