pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)
(Plasmid
#215010)
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PurposeExpress a Mitophagy reporter in mammalian cells (untested)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215010 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti UBC
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin ; mGFP
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepSu9 (Presequence of Fo-ATPase subunit 9)
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SpeciesOther
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Tag
/ Fusion Protein
- HaloTag(7)-mGFP (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP) was a gift from Wade Harper (Addgene plasmid # 215010 ; http://n2t.net/addgene:215010 ; RRID:Addgene_215010)