pKrox24(MapERK)d1EGFP (KroxDs)
(Plasmid #214912)

• Purpose: for PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activation
• Depositing Lab: Pavel Krejčí
• Publication: for PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activation (unpublished)

Backbone

• Vector backbone: pTR01F
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: d1EGFP under the MapERK promoter
• Species: Synthetic
• Promoter: MapkERK promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pKrox24(MapERK)d1EGFP (KroxDs).gb
• A portion of this plasmid was derived from a plasmid made by: The TR01F plasmid was obtained as a gift from Valeri Mossine (PMID: 24376882). The MapERK reporter was published previously and the sequence was also loaded to Addgene (#200112). d1EGFP DNA was obtained from Addgene (#47684).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry