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Addgene

SHIP2-N2
(Plasmid #214904)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214904 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV6-Entry-V5/His
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human SHIP2 aa 123-418
  • Alt name
    INPPL1
  • Species
    H. sapiens (human)
  • Mutation
    delta 1-122, 419-1258aa
  • Entrez Gene
    INPPL1 (a.k.a. OPSMD, SHIP2)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5/His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SHIP2-N2 was a gift from Pavel Krejčí (Addgene plasmid # 214904 ; http://n2t.net/addgene:214904 ; RRID:Addgene_214904)

  • For your References section:

    The inositol phosphatase SHIP2 enables sustained ERK activation downstream of FGF receptors by recruiting Src kinases. Fafilek B, Balek L, Bosakova MK, Varecha M, Nita A, Gregor T, Gudernova I, Krenova J, Ghosh S, Piskacek M, Jonatova L, Cernohorsky NH, Zieba JT, Kostas M, Haugsten EM, Wesche J, Erneux C, Trantirek L, Krakow D, Krejci P. Sci Signal. 2018 Sep 18;11(548):eaap8608. doi: 10.1126/scisignal.aap8608. 10.1126/scisignal.aap8608 PubMed 30228226
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