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pColdI-GST-mGTSF1
(Plasmid #214671)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214671 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pColdI-GST
  • Backbone manufacturer
    Takara
  • Total vector size (bp) 5097
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GTSF1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    501
  • GenBank ID
    NM_028797.1
  • Entrez Gene
    Gtsf1 (a.k.a. 1700006H03Rik, Cue110)
  • Promoter CspA
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • 6x His (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer acgccatatcgccgaaagg
  • 3′ sequencing primer ggcagggatcttagattctg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pColdI-GST-mGTSF1 was a gift from Phillip Zamore (Addgene plasmid # 214671 ; http://n2t.net/addgene:214671 ; RRID:Addgene_214671)

  • For your References section:

    GTSF1 accelerates target RNA cleavage by PIWI-clade Argonaute proteins. Arif A, Bailey S, Izumi N, Anzelon TA, Ozata DM, Andersson C, Gainetdinov I, MacRae IJ, Tomari Y, Zamore PD. Nature. 2022 Aug;608(7923):618-625. doi: 10.1038/s41586-022-05009-0. Epub 2022 Jun 30. 10.1038/s41586-022-05009-0 PubMed 35772669
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