pET23a-H6-TEV-cAbl
(Plasmid #214234)

• Purpose: Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings
• Depositing Lab: Neel Shah
• Publication: Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 10.7554/eLife.35190.

Backbone

• Vector backbone: pET23a
• Backbone size w/o insert (bp): 3700
• Total vector size (bp): 4519
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ABL1 Kinase domain
• Species: H. sapiens (human)
• Insert Size (bp): 819
• Entrez Gene: ABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 promotor

Resource Information

• Supplemental Documents:
  • Shah_H7Y_2_pET-23a-H6-TEV-Abl-KD_pLann.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This protein needs to be co-expressed in E. coli BL21(DE3) cells with the YopH tyrosine phosphatase