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pET23a-H6-TEV-cSrc
(Plasmid #214233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23a
  • Backbone size w/o insert (bp) 3700
  • Total vector size (bp) 4513
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    cSrc Kinase domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    813
  • Entrez Gene
    SRC (a.k.a. ASV, SRC1, THC6, c-SRC, p60-Src)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This protein needs to be co-expressed in E. coli BL21(DE3) cells with the YopH tyrosine phosphatase

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET23a-H6-TEV-cSrc was a gift from Neel Shah (Addgene plasmid # 214233 ; http://n2t.net/addgene:214233 ; RRID:Addgene_214233)

  • For your References section:

    Fine-tuning of substrate preferences of the Src-family kinase Lck revealed through a high-throughput specificity screen. Shah NH, Lobel M, Weiss A, Kuriyan J. Elife. 2018 Mar 16;7. pii: 35190. doi: 10.7554/eLife.35190. 10.7554/eLife.35190 PubMed 29547119
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