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Addgene

pLXSN-MRGPRX2
(Plasmid #213960)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213960 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLXSN
  • Backbone manufacturer
    Provided by the research group of Dr. R. Nicholas, University of North Carolina, USA
  • Backbone size w/o insert (bp) 5900
  • Vector type
    Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MRGPRX2
  • GenBank ID
    NM-054030 NM-054030
  • Entrez Gene
    MRGPRX2 (a.k.a. MGRG3, MRGX2)
  • Promoter LTR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer cgtaatGAATTCaccATGGATCCAACCACCCCGGCC
  • 3′ sequencing primer gcatataCTCGAGCTACACCAGACTGCTTCTCGAC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Christa Müller research group

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLXSN-MRGPRX2 was a gift from Christa Müller (Addgene plasmid # 213960 ; http://n2t.net/addgene:213960 ; RRID:Addgene_213960)

  • For your References section:

    Proinflammatory chemokine CXCL14 activates MAS-related G protein-coupled receptor MRGPRX2 and its putative mouse ortholog MRGPRB2. Al Hamwi G, Namasivayam V, Buschbell B, Gedschold R, Golz S, Muller CE. Commun Biol. 2024 Jan 6;7(1):52. doi: 10.1038/s42003-023-05739-5. 10.1038/s42003-023-05739-5 PubMed 38184723
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