pLXSN-MRGPRX2
(Plasmid #213960)

• Purpose: Expresses MRGPRX2 in mammalian cells
• Depositing Lab: Christa Müller
• Publication: Al Hamwi et al Commun Biol. 2024 Jan 6;7(1):52. doi: 10.1038/s42003-023-05739-5.

Backbone

• Vector backbone: pLXSN
• Backbone manufacturer: Provided by the research group of Dr. R. Nicholas, University of North Carolina, USA
• Backbone size w/o insert (bp): 5900
• Vector type: Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MRGPRX2
• GenBank ID: NM-054030 NM-054030
• Entrez Gene: MRGPRX2 (a.k.a. MGRG3, MRGX2)
• Promoter: LTR

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: cgtaatGAATTCaccATGGATCCAACCACCCCGGCC
• 3′ sequencing primer: gcatataCTCGAGCTACACCAGACTGCTTCTCGAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Christa Müller research group

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry