pLVX-IRES-MRGPRB2-mCherry
(Plasmid
#213961)
-
PurposeExpresses MRGPRB2 in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 213961 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLVX-IRES-mCherry
-
Backbone manufacturerClontech, Takara Bio Europe, Saint-Germain-en-Laye, France
- Backbone size w/o insert (bp) 8172
-
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameMRGPRB2
-
SpeciesM. musculus (mouse)
-
Entrez GeneMrgprb2 (a.k.a. 4833406I20Rik, Mgrg14)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer ccgCTCGAGaccATGTACCCCTACGACGTGCCCGACTACGCGACCGGTATGAGTGGAGATTTCCTAATCAAG
- 3′ sequencing primer ccgGGATCCTCAGCTGCAGCTCTGAACAG (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byChrista Müller research group
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLVX-IRES-MRGPRB2-mCherry was a gift from Christa Müller (Addgene plasmid # 213961 ; http://n2t.net/addgene:213961 ; RRID:Addgene_213961) -
For your References section:
Proinflammatory chemokine CXCL14 activates MAS-related G protein-coupled receptor MRGPRX2 and its putative mouse ortholog MRGPRB2. Al Hamwi G, Namasivayam V, Buschbell B, Gedschold R, Golz S, Muller CE. Commun Biol. 2024 Jan 6;7(1):52. doi: 10.1038/s42003-023-05739-5. 10.1038/s42003-023-05739-5 PubMed 38184723