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Addgene

pLVX-IRES-MRGPRB2-mCherry
(Plasmid #213961)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213961 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLVX-IRES-mCherry
  • Backbone manufacturer
    Clontech, Takara Bio Europe, Saint-Germain-en-Laye, France
  • Backbone size w/o insert (bp) 8172
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MRGPRB2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Mrgprb2 (a.k.a. 4833406I20Rik, Mgrg14)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer ccgCTCGAGaccATGTACCCCTACGACGTGCCCGACTACGCGACCGGTATGAGTGGAGATTTCCTAATCAAG
  • 3′ sequencing primer ccgGGATCCTCAGCTGCAGCTCTGAACAG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Christa Müller research group

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-IRES-MRGPRB2-mCherry was a gift from Christa Müller (Addgene plasmid # 213961 ; http://n2t.net/addgene:213961 ; RRID:Addgene_213961)
  • For your References section:

    Proinflammatory chemokine CXCL14 activates MAS-related G protein-coupled receptor MRGPRX2 and its putative mouse ortholog MRGPRB2. Al Hamwi G, Namasivayam V, Buschbell B, Gedschold R, Golz S, Muller CE. Commun Biol. 2024 Jan 6;7(1):52. doi: 10.1038/s42003-023-05739-5. 10.1038/s42003-023-05739-5 PubMed 38184723