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Addgene

p104_gRNA_Sp_CD3e
(Plasmid #213780)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213780 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiviral
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sp gRNA
  • gRNA/shRNA sequence
    GCCAAGTGAGGTAAAACCCGC

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p104_gRNA_Sp_CD3e was a gift from Howard Chang (Addgene plasmid # 213780 ; http://n2t.net/addgene:213780 ; RRID:Addgene_213780)

  • For your References section:

    Bidirectional epigenetic editing reveals hierarchies in gene regulation. Pacalin NM, Steinhart Z, Shi Q, Belk JA, Dorovskyi D, Kraft K, Parker KR, Shy BR, Marson A, Chang HY. Nat Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02213-3. 10.1038/s41587-024-02213-3 PubMed 38760566
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