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Addgene

pRK5-PLA2G15-6xHis S198A
(Plasmid #213605)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213605 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Phospholipase A2 Group XV
  • Alt name
    PLA2G15
  • Alt name
    Lysosomal phospholipase A2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1325
  • Mutation
    Changed serine 198 to alanine
  • GenBank ID
    23659
  • Entrez Gene
    PLA2G15 (a.k.a. ACS, GXVPLA2, LLPL, LPLA2, LYPLA3)
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCCCAGGTCCAACTGCACCTCGGTTCTATCGATTGAATTC
  • 3′ sequencing primer GCGGCCGCTAAGTAAGTAAGGATCCCCAGCTTGGCCGCCA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-PLA2G15-6xHis S198A was a gift from Monther Abu-Remaileh (Addgene plasmid # 213605 ; http://n2t.net/addgene:213605 ; RRID:Addgene_213605)

  • For your References section:

    Glycerophosphodiesters inhibit lysosomal phospholipid catabolism in Batten disease. Nyame K., Hims, A., Aburous, A., Laqtom, N. N., Dong, W., Medoh, U. N., Heiby, J. C., Xiong, J., Ori, A., & Abu-Remaileh, M.. Molecular Cell, March 5 2024 10.1016/j.molcel.2024.02.006
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