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pAAV-CMV-SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA
(Plasmid #213536)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213536 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 3799
  • Total vector size (bp) 6475
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA-HA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2676
  • Promoter CMV
  • Tags / Fusion Proteins
    • EGFP (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HinDIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer CAACAGATGGCTGGCAACTAGAAGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CMV-SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA was a gift from Wenjing Wang (Addgene plasmid # 213536 ; http://n2t.net/addgene:213536 ; RRID:Addgene_213536)

  • For your References section:

    A general method for chemogenetic control of peptide function. Shen J, Geng L, Li X, Emery C, Kroning K, Shingles G, Lee K, Heyden M, Li P, Wang W. Nat Methods. 2023 Jan;20(1):112-122. doi: 10.1038/s41592-022-01697-8. Epub 2022 Dec 8. 10.1038/s41592-022-01697-8 PubMed 36481965
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