pLew100::NLS-ISceI-HA
(Plasmid #21299)

• Depositing Lab: Nina Papavasiliou
• Publication: Boothroyd et al Nature. 2009 Apr 15. ():.

Backbone

• Vector backbone: pLew100
• Backbone size w/o insert (bp): 4300
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: I-SceI
• Insert Size (bp): 2000
• GenBank ID: FJ797515
• Entrez Gene: SCEI (a.k.a. Q0160)
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are a few discrepancies between Addgene's sequence and the provided sequence from the depositing lab. These discrepancies do not affect plasmid function.