pBabe hygro MTH1
(Plasmid
#21296)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBABE-hygro
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Backbone manufacturerAvailable at Addgene (#1765)
- Backbone size w/o insert (bp) 5169
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Vector typeMammalian Expression, Retroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMTH1
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SpeciesH. sapiens (human)
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Entrez GeneNUDT1 (a.k.a. MTH1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Yusaku Nakabeppu provided pcDEB.MTH1
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe hygro MTH1 was a gift from Bob Weinberg (Addgene plasmid # 21296 ; http://n2t.net/addgene:21296 ; RRID:Addgene_21296) -
For your References section:
Continuous elimination of oxidized nucleotides is necessary to prevent rapid onset of cellular senescence. Rai P, Onder TT, Young JJ, McFaline JL, Pang B, Dedon PC, Weinberg RA. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):169-74. 10.1073/pnas.0809834106 PubMed 19118192