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Addgene

pBigT
(Plasmid #21270)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21270 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart
Cloning Grade DNA 21270-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $105

Backbone

  • Vector backbone
    pBigT
  • Backbone manufacturer
    Costantini Lab
  • Backbone size (bp) 6117
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer M13-fwd
  • 3′ sequencing primer M13-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBigT is used for making transgenic mice. The plasmid contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence. See article for detailed information regarding the creation and use of this plasmid.

Information for Cloning Grade DNA (Catalog # 21270-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $105 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBigT was a gift from Frank Costantini (Addgene plasmid # 21270 ; http://n2t.net/addgene:21270 ; RRID:Addgene_21270)

  • For your References section:

    Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, Costantini F. BMC Dev Biol. 2001 . 1():4. 10.1186/1471-213X-1-4 PubMed 11299042
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