MSCV JMJD3 mutant
(Plasmid #21214)

• Depositing Lab: Paul Khavari
• Publication: Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

Backbone

• Vector backbone: pMSCVpuro
• Backbone size w/o insert (bp): 6300
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: JMJD3
• Species: H. sapiens (human)
• Mutation: H1390A
• Entrez Gene: KDM6B (a.k.a. JMJD3, NEDCFSA, NEDSST)
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLXSN-5
• 3′ sequencing primer: MSCV-rev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry