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Addgene

PM-GFP
(Plasmid #21213)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21213 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5419
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PM-GFP
  • Alt name
    GFP-pcDNA3-LYN
  • Insert Size (bp)
    761
  • Mutation
    PM targetting sequence is Lyn (MGCIKSKRKD) (Lab plasmid WC 0017)
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PM-GFP was a gift from Tobias Meyer (Addgene plasmid # 21213 ; http://n2t.net/addgene:21213 ; RRID:Addgene_21213)

  • For your References section:

    A versatile microporation technique for the transfection of cultured CNS neurons. Teruel MN, Blanpied TA, Shen K, Augustine GJ, Meyer T. J Neurosci Methods. 1999 Oct 30. 93(1):37-48. 10.1016/S0165-0270(99)00112-0 PubMed 10598863
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