pRRL-VinculinVenus-S1033D
(Plasmid #211897)

• Purpose: Vinculin (chicken) with S1033 phosphomimetic point mutation (S1033D) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.
• Depositing Lab: Brenton Hoffman
• Publication: Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;120(50):e2316456120. doi: 10.1073/pnas.2316456120. Epub 2023 Dec 6.

Backbone

• Vector backbone: pRRL
• Backbone size w/o insert (bp): 6129
• Total vector size (bp): 10778
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: VinculinVenus-S1033D
• Alt name: VinV-S1033D
• Species: G. gallus (chicken)
• Insert Size (bp): 4649
• Mutation: mutated vinculin serine 1033 to aspartic acid (S1033D), creates phosphomimetic mutant
• Entrez Gene: VCL (a.k.a. VINC1)
• Promoter: CMV
• Tag / Fusion Protein:
  • VenusA206K (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: GGTACAGTGCAGGGGAAAG
• 3′ sequencing primer: GTTAAGAATACCAGTCAATCTTTCAC

Resource Information

• Supplemental Documents:
  • prrl_vinculin_venus_s1033d.gb
• A portion of this plasmid was derived from a plasmid made by: Martin Schwartz (Addgene #27300)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry