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Addgene

EC67908
(Plasmid #211795)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211795 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PICSL4723
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HptII, Cas12a, GR-GRF-GIF

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BpiI (destroyed during cloning)
  • 3′ cloning site BpiI (destroyed during cloning)
  • 5′ sequencing primer whole plasmid sequencing
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EC67908 was a gift from Wendy Harwood & Cristobal Uauy (Addgene plasmid # 211795 ; http://n2t.net/addgene:211795 ; RRID:Addgene_211795)

  • For your References section:

    An Optimised CRISPR Cas9 and Cas12a Toolkit for Barley and Wheat. Lawrenson T, Clarke M, Kirby R, Forner M, Steuernagel B, Harwood W. Research Square, 12 March 2024, PREPRINT 10.21203/rs.3.rs-4024107/v1
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