pSIN4-CMV-K2M
(Plasmid #21164)

• Depositing Lab: James Thomson
• Publication: Yu et al Science. 2009 Mar 26.

Backbone

• Vector backbone: pSIN4-EF2-IRES-Pur
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression, Lentiviral ; reprogramming

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Klf4, cMyc
• Species: H. sapiens (human)
• Insert Size (bp): 2733
• GenBank ID: NM_004235.2 NM_002467
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
• Entrez Gene: KLF4 (a.k.a. EZF, GKLF)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: EF1a_for

Resource Information

• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

After lentivirus infection, Klf4 and cMyc are coexpressed by CMV promoter and IRES intron. Infections with this lentivirus, when used with pSIN4-EF2-O2S and pSIN4-EF2-N2L have a significantly greater reprogramming efficiency than our original 4 lentiviruses.

NOTE: Addgene's quality control sequence found a K269T in Klf4. This mutation is not known to affect function.