pSIN4-EF2-N2L
(Plasmid #21163)

• Depositing Lab: James Thomson
• Publication: Yu et al Science. 2009 Mar 26.

Backbone

• Vector backbone: pSIN4-EF2-IRES-Pur
• Backbone size w/o insert (bp): 6300
• Vector type: Mammalian Expression, Lentiviral ; reprogramming

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Nanog, Lin28
• Species: H. sapiens (human)
• Insert Size (bp): 1548
• GenBank ID: NM_024865 NM_024674
• Entrez Gene: NANOG
• Entrez Gene: LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (unknown if destroyed)
• 5′ sequencing primer: EF1a_for

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

After lentivirus infection, Nanog and Lin28 are coexpressed by IRES2 intron. Infection with this lentivirus, when used with pSIN4-EF2-O2S and pSIN4-CMV-K2M have a significantly greater reprogramming efficiency than our original 4 lentiviruses.