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Addgene

TRE-eGFP-F1A
(Plasmid #211458)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211458 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-BSD-tet
  • Backbone size w/o insert (bp) 8011
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flotillin-1-palmitoylation mutant control peptide
  • Alt name
    F1A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    54
  • Mutation
    C34A
  • GenBank ID
    NM_005803.4 NM_005803.4
  • Entrez Gene
    FLOT1
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TATGTCGAGGTAGGCGTGTA
  • 3′ sequencing primer ctctaggcaccggatcaatt
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    gene was delivered as a gBlock from IDT with 40bp homology ends compatible with gibson cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sequence for F1A: ATGGTGGCTGGAGGGCGTGTCTTTGTCCTGCCCGCAATCCAACAGATCCAGAGGATCTAA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TRE-eGFP-F1A was a gift from Linda DeGraffenried (Addgene plasmid # 211458 ; http://n2t.net/addgene:211458 ; RRID:Addgene_211458)

  • For your References section:

    Flotillin-1 palmitoylation is essential for its stability and subsequent tumor promoting capabilities. McClellan B, Wilson CN, Brenner AJ, Jolly CA, deGraffenried L. Oncogene. 2024 Mar;43(14):1063-1074. doi: 10.1038/s41388-024-02946-0. Epub 2024 Feb 19. 10.1038/s41388-024-02946-0 PubMed 38374406
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