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Addgene

pcDNA3-TopBP1-mCer
(Plasmid #211414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-mCer
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TopBP1
  • Species
    H. sapiens (human)
  • Entrez Gene
    TOPBP1 (a.k.a. Dpb11, TOP2BP1)
  • Tag / Fusion Protein
    • mCerulean (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

generated by Julia Kutz (Leibniz Institute on Aging - Fritz Lipmann Institute, D-07745 Jena, Germany)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-TopBP1-mCer was a gift from Helmut Pospiech (Addgene plasmid # 211414 ; http://n2t.net/addgene:211414 ; RRID:Addgene_211414)
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