pSL5200 (pDonor_ISGst3)
(Plasmid
#210875)
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PurposeHarbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 210875 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDFDuet-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameISGst3
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SpeciesGeobacillus Stearothermophilus
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSL5200 (pDonor_ISGst3) was a gift from Samuel H. Sternberg (Addgene plasmid # 210875 ; http://n2t.net/addgene:210875 ; RRID:Addgene_210875) -
For your References section:
Transposon-encoded nucleases use guide RNAs to promote their selfish spread. Meers C, Le HC, Pesari SR, Hoffmann FT, Walker MWG, Gezelle J, Tang S, Sternberg SH. Nature. 2023 Sep 27. doi: 10.1038/s41586-023-06597-1. 10.1038/s41586-023-06597-1 PubMed 37758954
