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Addgene

pSL4164 (pEffector_IscB,ωRNA)
(Plasmid #210874)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210874 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCDFDuet-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GstIscB
  • Species
    Geobacillus Stearothermophilus

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL4164 (pEffector_IscB,ωRNA) was a gift from Samuel H. Sternberg (Addgene plasmid # 210874 ; http://n2t.net/addgene:210874 ; RRID:Addgene_210874)
  • For your References section:

    Transposon-encoded nucleases use guide RNAs to promote their selfish spread. Meers C, Le HC, Pesari SR, Hoffmann FT, Walker MWG, Gezelle J, Tang S, Sternberg SH. Nature. 2023 Sep 27. doi: 10.1038/s41586-023-06597-1. 10.1038/s41586-023-06597-1 PubMed 37758954