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pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro
(Plasmid #210857)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210857 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH1-CMV-MCS-SV40-Hygro
  • Vector type
    Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FIP200
  • Species
    H. sapiens (human)
  • Mutation
    V44D/V314D/L324D/R543D/R548D
  • Entrez Gene
    RB1CC1 (a.k.a. ATG17, CC1, FIP200, PPP1R131)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro was a gift from James Hurley (Addgene plasmid # 210857 ; http://n2t.net/addgene:210857 ; RRID:Addgene_210857)

  • For your References section:

    Structure and activation of the human autophagy-initiating ULK1C:PI3KC3-C1 supercomplex. Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH. bioRxiv 2023.06.01.543278 10.1101/2023.06.01.543278
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