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Addgene

pET28sumo-hRig-I-Full-length
(Plasmid #210827)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210827 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet28-sumo
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hRIG-I (DDX58) Full length protein
  • Species
    H. sapiens (human)
  • Mutation
    NA
  • Entrez Gene
    RIGI (a.k.a. DDX58, RIG-I, RIG1, RLR-1, SGMRT2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28sumo-hRig-I-Full-length was a gift from Anna Pyle (Addgene plasmid # 210827 ; http://n2t.net/addgene:210827 ; RRID:Addgene_210827)

  • For your References section:

    Visualizing the determinants of viral RNA recognition by innate immune sensor RIG-I. Luo D, Kohlway A, Vela A, Pyle AM. Structure. 2012 Nov 7;20(11):1983-8. doi: 10.1016/j.str.2012.08.029. Epub 2012 Sep 27. 10.1016/j.str.2012.08.029 PubMed 23022350
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