pmStrawberry-Atg4BC74A
(Plasmid #21076)

• Depositing Lab: Tamotsu Yoshimori
• Publication: Fujita et al Mol Biol Cell. 2008 Nov . 19(11):4651-9.

Backbone

• Vector backbone: pmStrawberry C1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Atg4B(C74A)
• Alt name: Atg4B
• Species: M. musculus (mouse)
• Insert Size (bp): 1194
• Mutation: 220-222 TGC(Cys) is changed to GCA (Ala)
• GenBank ID: NM_174874
• Entrez Gene: Atg4b (a.k.a. 2510009N07Rik, Apg4b, Atg4bl, Autl1, MmAPG4B)
• Tag / Fusion Protein:
  • mStrawberry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21.
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.

Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Nat Biotechnol. 2004 Dec;22(12):1567-72.

Atg4B insert also contains V190A and T325K compared to listed reference sequence.