Skip to main content
Addgene

pESC-URA-Rnq1-GFP
(Plasmid #21051)

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21051 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pESC-URA
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Yeast Expression ; yeast/bacteria shuttle
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rnq1
  • Alt name
    YCL028W
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1218
  • Entrez Gene
    RNQ1 (a.k.a. YCL028W)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer ATTTTCGGTTTGTATTACTTC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pESC-URA-Rnq1-GFP was a gift from Judith Frydman (Addgene plasmid # 21051 ; http://n2t.net/addgene:21051 ; RRID:Addgene_21051)
  • For your References section:

    Misfolded proteins partition between two distinct quality control compartments. Kaganovich D, Kopito R, Frydman J. Nature. 2008 Aug 28. 454(7208):1088-95. 10.1038/nature07195 PubMed 18756251