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pmRFP_LAP2beta_IRES_puro2b
(Plasmid #21047)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21047 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    IRES_puro2b
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LAP2_beta
  • Alt name
    Tmpo
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    TMPO (a.k.a. CMD1T, LAP2, LEMD4, PRO0868, TP)
  • Tag / Fusion Protein
    • mRFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site - (unknown if destroyed)
  • 3′ cloning site - (unknown if destroyed)
  • 5′ sequencing primer -
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmRFP_LAP2beta_IRES_puro2b was a gift from Daniel Gerlich (Addgene plasmid # 21047 ; http://n2t.net/addgene:21047 ; RRID:Addgene_21047)

  • For your References section:

    Aurora B-mediated abscission checkpoint protects against tetraploidization. Steigemann P, Wurzenberger C, Schmitz MH, Held M, Guizetti J, Maar S, Gerlich DW. Cell. 2009 Feb 6. 136(3):473-84. 10.1016/j.cell.2008.12.020 PubMed 19203582
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