-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 21045 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneIRES_puro_2b
- Backbone size w/o insert (bp) 5835
-
Vector typeMammalian Expression, Bacterial Expression
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namepH2B
-
SpeciesH. sapiens (human)
-
Insert Size (bp)389
-
GenBank IDNM_021058
-
Entrez GeneH2BC16P (a.k.a. H2BFIP, HIST1H2BPS2, pH2B.i, pH2B/i)
-
Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site - (unknown if destroyed)
- 3′ cloning site - (unknown if destroyed)
- 5′ sequencing primer - (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pH2B_mCherry_IRES_puro2 was a gift from Daniel Gerlich (Addgene plasmid # 21045 ; http://n2t.net/addgene:21045 ; RRID:Addgene_21045) -
For your References section:
Aurora B-mediated abscission checkpoint protects against tetraploidization. Steigemann P, Wurzenberger C, Schmitz MH, Held M, Guizetti J, Maar S, Gerlich DW. Cell. 2009 Feb 6. 136(3):473-84. 10.1016/j.cell.2008.12.020 PubMed 19203582