pH2B_mCherry_IRES_neo3
(Plasmid #21044)

• Depositing Lab: Daniel Gerlich
• Publication: Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Backbone

• Vector backbone: IRES_neo3
• Backbone size w/o insert (bp): 5903
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pH2B
• Species: H. sapiens (human)
• Insert Size (bp): 389
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: - (unknown if destroyed)
• 3′ cloning site: - (unknown if destroyed)
• 5′ sequencing primer: -

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry