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Addgene

pETSUMO-TtAgocatdead
(Plasmid #210322)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210322 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETSUMO
  • Backbone size w/o insert (bp) 5643
  • Total vector size (bp) 7701
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TtAgo
  • Alt name
    TT_P0026
  • Species
    Thermus thermophilus
  • Insert Size (bp)
    2057
  • Entrez Gene
    TT_P0026 (a.k.a. TT_P0026, TTP0026)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6x His tag (N terminal on backbone)
    • SUMO tag (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETSUMO-TtAgocatdead was a gift from Phillip Zamore (Addgene plasmid # 210322 ; http://n2t.net/addgene:210322 ; RRID:Addgene_210322)

  • For your References section:

    Thermus thermophilus Argonaute Functions in the Completion of DNA Replication. Jolly SM, Gainetdinov I, Jouravleva K, Zhang H, Strittmatter L, Bailey SM, Hendricks GM, Dhabaria A, Ueberheide B, Zamore PD. Cell. 2020 Sep 17;182(6):1545-1559.e18. doi: 10.1016/j.cell.2020.07.036. Epub 2020 Aug 25. 10.1016/j.cell.2020.07.036 PubMed 32846159
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