pcDNA5-FRT-TO-mCherry-N
(Plasmid
#210271)
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Purpose(Empty Backbone) N-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 210271 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5/FRT/TO
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersHygromycin ; Blasticidin
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Tag
/ Fusion Protein
- mCherry (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- mCherry (N terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5-FRT-TO-mCherry-N was a gift from Niels Mailand (Addgene plasmid # 210271 ; http://n2t.net/addgene:210271 ; RRID:Addgene_210271) -
For your References section:
The SUMO-NIP45 pathway processes toxic DNA catenanes to prevent mitotic failure. Hertz EPT, Vega IA, Kruse T, Wang Y, Hendriks IA, Bizard AH, Eugui-Anta A, Hay RT, Nielsen ML, Nilsson J, Hickson ID, Mailand N. Nat Struct Mol Biol. 2023 Jul 20. doi: 10.1038/s41594-023-01045-0. 10.1038/s41594-023-01045-0 PubMed 37474739
