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Addgene

EGFP-NIP45 D394R
(Plasmid #210265)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210265 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO/EGFP-N
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin ; Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NIP45 D394R siRES
  • Species
    Synthetic
  • Mutation
    D394R, siRES
  • Entrez Gene
    NFATC2IP (a.k.a. ESC2, NIP45, RAD60)
  • Promoter CMV/TetO2
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-NIP45 D394R was a gift from Niels Mailand (Addgene plasmid # 210265 ; http://n2t.net/addgene:210265 ; RRID:Addgene_210265)

  • For your References section:

    The SUMO-NIP45 pathway processes toxic DNA catenanes to prevent mitotic failure. Hertz EPT, Vega IA, Kruse T, Wang Y, Hendriks IA, Bizard AH, Eugui-Anta A, Hay RT, Nielsen ML, Nilsson J, Hickson ID, Mailand N. Nat Struct Mol Biol. 2023 Jul 20. doi: 10.1038/s41594-023-01045-0. 10.1038/s41594-023-01045-0 PubMed 37474739
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