pCXLE-Sox2AV-T2A-Klf4-E2A-Myc
(Plasmid
#210017)
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Purposeepisomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for high-quality integration-free naïve reset; best for rodents and primates (including human).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210017 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCXLE
- Backbone size w/o insert (bp) 10180
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSox2AV-t2a-Klf4-e2a-cMyc
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Alt nameKlf4
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Alt nameSox2
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Alt nameMyc
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SpeciesM. musculus (mouse)
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Insert Size (bp)3955
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MutationSox2A61V is a point mutant of Sox2 that enhances its dimerization with POU factors, such as Oct4
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Entrez GeneKlf4 (a.k.a. EZF, Gklf, Zie)
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Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer na
- 3′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Deliver to your PSCs combined with pCXWB-EBNA1 (Addgene #37624)
Please visit https://www.biorxiv.org/content/10.1101/2022.09.23.509242v1 for BioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCXLE-Sox2AV-T2A-Klf4-E2A-Myc was a gift from Hans Schöler (Addgene plasmid # 210017 ; http://n2t.net/addgene:210017 ; RRID:Addgene_210017) -
For your References section:
Highly cooperative chimeric super-SOX induces naive pluripotency across species. MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, Velychko S. Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 10.1016/j.stem.2023.11.010. Epub 2023 Dec 22. 10.1016/j.stem.2023.11.010 PubMed 38141611