H2B-mCherry
(Plasmid #20972)

• Purpose: H2B fused to mCherry for chromatin visualization
• Depositing Lab: Robert Benezra
• Publication: Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Histone cluster 1, H2bb
• Alt name: H2B.1
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• GenBank ID: NM_021062
• Entrez Gene: H2BC3 (a.k.a. H2B.1, H2B/f, H2BFF, HIST1H2BB)
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• Supplemental Documents:
  • H2B-mCherry GenBank
• Articles Citing this Plasmid:
  • 76 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139) The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3) That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The mCherry cDNA was obtained from Dr. Roger Tsien.