mId1-Venus
(Plasmid #20966)

• Depositing Lab: Robert Benezra
• Publication: Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Inhibitor of DNA binding 1
• Alt name: Id1
• Species: M. musculus (mouse)
• Insert Size (bp): 500
• GenBank ID: NM_010495
• Entrez Gene: Id1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)
• Tag / Fusion Protein:
  • Venus (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• Supplemental Documents:
  • Annotated Depositor's Sequence
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 10-amino acid linker between the mouse Id1 and VenusYFP, GSAGSAAGSG, is derived from a linker described in Waldo et al., 1999 (https://www.ncbi.nlm.nih.gov/pubmed/10404163) The Venus cDNA was obtained from Dr. Anna-Katerina Hadjantonakis.