Anti-Integrin alpha 5 beta 1 [IPI-ITGA5/ITGB1.2]
(Antibody
#209611)
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PurposeAnti-Integrin α5β1 (Human) chimeric recombinant antibody with fused human variable and rabbit constant domains; binds the particular alpha/beta chain pair, does not block function
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Depositing Lab
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Recommended Applications
Image 1 (of 3): Flow Cytometry
Bar diagram showing mean fluorescence intensity (MFI) from flow cytometry analysis on Exp…
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Credit: Institute for Protein Innovation (IPI)
Image 2 (of 3): Flow Cytometry
Bar diagram showing mean fluorescence intensity (MFI) from flow cytometry analysis on Exp…
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Credit: Institute for Protein Innovation (IPI)
Image 3 (of 3): Flow Cytometry
Histogram from FACS analysis on Expi293F α5-/αV- cells (blue) and Expi293F α5-/αV- transi…
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Credit: Institute for Protein Innovation (IPI)
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Recombinant Antibody | 209611-rAb | 100 µg of purified recombinant antibody | $250 * | ||
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Features
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Source SpeciesO. cuniculus (rabbit)
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IsotypeIgG1
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Light Chain Typekappa
Production & Usage
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Recommended Secondary AntibodyAnti-rabbit IgG
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PurificationPurification from cell culture supernatant using affinity chromatography followed by buffer exchange with centrifugal columns.
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Storage BufferPBS
Delivery
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Concentration0.9–1.1 mg/mL
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StorageUpon receipt, aliquot and store at 4 °C until use.
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ShipmentBlue ice
Resource Information
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Persistent Resource IdentifierRRID:AB_3076497
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Zeocin® is an InvivoGen trademark.
Trademarks:
Target Antigen (2)
| Protein | Integrin beta-1 |
|---|---|
| Antigen Description | Ectodomain of human β1 integrin |
| Species | H. sapiens (human) |
| Additional Species Reactivity | M. musculus (mouse) |
| Gene | ITGB1 |
| Alternative Names |
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| External References |
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| Protein | Integrin alpha-5 |
|---|---|
| Antigen Description | Ectodomain of human α5 chain |
| Species | H. sapiens (human) |
| Additional Species Reactivity | M. musculus (mouse) |
| Gene | ITGA5 |
| Alternative Names |
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| External References |
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Applications (1)
Flow Cytometry
Depositor Comments
These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Anti-Integrin alpha 5 beta 1 [IPI-ITGA5/ITGB1.2] - from Institute for Protein Innovation (IPI) (Addgene antibody # 209611 ; http://n2t.net/addgene:209611 ; RRID:AB_3076497) -
For your References section:
Single-molecule characterization of subtype-specific beta1 integrin mechanics. Jo MH, Li J, Jaumouille V, Hao Y, Coppola J, Yan J, Waterman CM, Springer TA, Ha T. Nat Commun. 2022 Dec 3;13(1):7471. doi: 10.1038/s41467-022-35173-w. 10.1038/s41467-022-35173-w PubMed 36463259
IPI Antibody Collection
This antibody was developed by the Institute for Protein Innovation (IPI).
Learn about the IPI Collection
The heavy chain and light chain amino acid sequences are available by request after purchase. Please submit requests to [email protected]. For all other questions, please send an email to [email protected] for assistance.
Please see the bioRxiv preprint (Link opens in a new window) for antibody characterization and applications and Jo et al. (2022) (Link opens in a new window) for further characterization and applications.