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Addgene

PB-CA
(Plasmid #20960)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20960 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-CA
  • Backbone size w/o insert (bp) 6465
  • Vector type
    Mammalian Expression ; piggyBac

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdB survival cells
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gateway Destination Vector
  • Insert Size (bp)
    1452

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 (not destroyed)
  • 3′ cloning site attR2 (not destroyed)
  • 5′ sequencing primer M13 R, T3
  • 3′ sequencing primer M13F, T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    piggyBac terminal repeats obtained from Pentao Liu at Sanger
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-CA was a gift from Andras Nagy (Addgene plasmid # 20960 ; http://n2t.net/addgene:20960 ; RRID:Addgene_20960)

  • For your References section:

    piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hämäläinen R, Cowling R, Wang W, Liu P, Gertsenstein M, Kaji K, Sung HK, Nagy A. Nature. 2009 Mar 1. ():. 10.1038/nature07863 PubMed 19252478
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