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Addgene

EYFP-Clathrin
(Plasmid #20921)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20921 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/V5-His TOPO
  • Backbone manufacturer
    Invitrogen cat#K480040
  • Backbone size w/o insert (bp) 5523
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Clathrin, light polypeptide (Lca)
  • Alt name
    Clathrin-Lca
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1058
  • GenBank ID
    BC057660
  • Entrez Gene
    Clta (a.k.a. Lca)
  • Tag / Fusion Protein
    • EYFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site AgeI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse priming site
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    J.H. Keen Nat.Cell Biol. 1,1,(1999)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EYFP-Clathrin was a gift from Xiaowei Zhuang (Addgene plasmid # 20921 ; http://n2t.net/addgene:20921 ; RRID:Addgene_20921)

  • For your References section:

    Assembly of endocytic machinery around individual influenza viruses during viral entry. Rust MJ, Lakadamyali M, Zhang F, Zhuang X. Nat Struct Mol Biol. 2004 Jun . 11(6):567-73. 10.1038/nsmb769 PubMed 15122347
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