pISV-CRISPR/Cas9PGmUbi
(Plasmid #209189)

• Purpose: Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection
• Depositing Lab: Christian Staehelin
• Publication: Christian Staehelin Lab Plasmids 2023 (unpublished)

Backbone

• Vector backbone: pISV-DsRed1
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Cas9
• Species: S. pyogenes
• Insert Size (bp): 6400
• Mutation: plant-codon optimized with higher GC contents (62.5%) in the 5' region (400 bp) and 54.2% overall GC content
• Promoter: GmUbi from Glycine max (L.) Merr. cv. Willams 82 (soybean)
• Tags / Fusion Proteins:
  • NLS (C terminal on insert)
  • NLS (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: DsRed(deltaB)
• Species: Synthetic
• Mutation: missing BsaI site
• Promoter: CaMV 35S

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pISV- CRISPRCas9PGmUbi.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry