PB-CA-rtTA Adv
(Plasmid #20910)

• Purpose: piggyBac vector for expression of reverse tetracycline transactivator from a CAG constitutive promoter
• Depositing Lab: Andras Nagy
• Publication: Woltjen et al Nature. 2009 Mar 1. ():.

Backbone

• Vector backbone: PB-CA
• Backbone size w/o insert (bp): 6262
• Vector type: Mammalian Expression ; piggyBac

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: rtTA-ON-Adv
• Insert Size (bp): 754

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: attB1 (not destroyed)
• 3′ cloning site: attB2 (not destroyed)
• 5′ sequencing primer: M13 R, T3
• 3′ sequencing primer: M13F, T7

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: piggyBac terminal repeats obtained from Pentao Liu at Sanger, CAG-rtTA Advanced obtained from Clontech.
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry