PB-TET
(Plasmid #20909)

• Purpose: piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)
• Depositing Lab: Andras Nagy
• Publication: Woltjen et al Nature. 2009 Mar 1. ():.

Backbone

• Vector backbone: pBluescript KS
• Backbone size w/o insert (bp): 9370
• Vector type: Mammalian Expression ; piggyBac
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: ccdB survival cells
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gateway Destination Vector
• Insert Size (bp): 1455
• Tag / Fusion Protein:
  • IRES-bGeo (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AttR1 (not destroyed)
• 3′ cloning site: AttR2 (not destroyed)
• 5′ sequencing primer: M13 F, T7
• 3′ sequencing primer: M13 R, T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: piggyBac terminal repeats obtained from Pentao Liu at Sanger.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry