piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)
(Plasmid #209086)

• Purpose: Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression
• Depositing Lab: Vikram Khurana
• Publication: Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33. doi: 10.1016/j.cell.2022.05.008.

Backbone

• Vector backbone: piggyBac-rtTA (4th_Gen)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: SNCA
• Species: H. sapiens (human)
• Entrez Gene: SNCA (a.k.a. NACP, PARK1, PARK4, PD1)
• Promoter: TRE4G
• Tag / Fusion Protein:
  • AviTag (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: NGN2
• Species: M. musculus (mouse)
• Entrez Gene: Neurog2 (a.k.a. Atoh4, Math4A, bHLHa8, ngn-2, ngn2)

Gene/Insert 3

• Gene/Insert name: BirA
• Species: E. coli
• Mutation: human codon optimized
• Promoter: hSyn

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pEHA1628_pB_TET_GW_Syn_Avi_NGN2_BirA.dna

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pEHA1628