Skip to main content
Addgene

pMSCV-FlagMLL2-pL-ENL(5613)
(Plasmid #20874)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20874 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMSCV-neo
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MLL2
  • Alt name
    MLLT1
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Entrez Gene
    Kmt2d (a.k.a. ALR, C430014K11Rik, KMT2B, Mll2, Mll4)
  • Entrez Gene
    MLLT1 (a.k.a. ENL, LTG19, YEATS1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer MSCV
  • 3′ sequencing primer MSCV-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

0.4kb NruI/XhoI fragment from pMSCV-flagMLL-pl-ENL(5613) was inserted into a NruI/XhoI cut pMSCV-flagMLL2pl vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-FlagMLL2-pL-ENL(5613) was a gift from Robert Slany (Addgene plasmid # 20874 ; http://n2t.net/addgene:20874 ; RRID:Addgene_20874)

  • For your References section:

    Alterations of the CxxC domain preclude oncogenic activation of mixed-lineage leukemia 2. Bach C, Mueller D, Buhl S, Garcia-Cuellar MP, Slany RK. Oncogene. 2009 Feb 12. 28(6):815-23. 10.1038/onc.2008.443 PubMed 19060922
Related items:
Commonly requested with: