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Addgene

pEGFP-dnMCAK
(Plasmid #208650)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208650 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    eGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Kif2c
  • Alt name
    MCAK
  • Species
    Cricetulus griseus (Chinese hamster)
  • Mutation
    S92A , S106A , S108A, S112A , S186A, H530A, R534A, and K537A
  • GenBank ID
    NM_001246742.1
  • Entrez Gene
    Kif2c (a.k.a. KNSL6, MCAK)
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-dnMCAK was a gift from Samuel Bakhoum (Addgene plasmid # 208650 ; http://n2t.net/addgene:208650 ; RRID:Addgene_208650)

  • For your References section:

    Non-cell-autonomous cancer progression from chromosomal instability. Li J, Hubisz MJ, Earlie EM, Duran MA, Hong C, Varela AA, Lettera E, Deyell M, Tavora B, Havel JJ, Phyu SM, Amin AD, Budre K, Kamiya E, Cavallo JA, Garris C, Powell S, Reis-Filho JS, Wen H, Bettigole S, Khan AJ, Izar B, Parkes EE, Laughney AM, Bakhoum SF. Nature. 2023 Aug;620(7976):1080-1088. doi: 10.1038/s41586-023-06464-z. Epub 2023 Aug 23. 10.1038/s41586-023-06464-z PubMed 37612508
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