Skip to main content
Addgene

FNLCR CRISPRa Cell Surface Libraries
(Pooled Libraries #207471, #1000000228)

Print
  • Purpose

    Two CRISPR activation libraries have been generated at the Frederick National Laboratory for Cancer Research (FNLCR) for screening human receptor-ligand interactions. The FNLCR CRISPRa Cell Surface Library includes guide RNAs targeting all known human cell surface proteins plus non-targeting controls. The combined FNLCR CRISPRa Cell Surface and Soluble Library includes all the Cell Surface Library gRNAs plus additional gRNAs targeting all known soluble factors. These libraries can be used to identify uncharacterized receptor-ligand pairings by activating the expression of the receptor when using a ligand as a bait.

  • Vector Backbone

    pGMC00029 (aka pRC0610) (Plate #200834) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 207471 FNLCR CRISPRa Cell Surface Library† 1 $540 Add to Cart
Pooled Library 1000000228 FNLCR CRISPRa Cell Surface and Soluble Libraries† 1 $750 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with lenti dCAS-VP64_Blast (Addgene #61425) and pGMC00030 (aka pRC0707) (Addgene #209673) or lentiMPH v2 (Addgene #89308), or other plasmids or cell lines expressing both dCas9-VP64 and MS2-P65-HSF1.

Library Details

  • Species
    Human
  • Genes targeted


    Cell Surface Library: 6,081
    Soluble Library: 949

  • gRNAs


    Cell Surface Library: 30,868
    Soluble Library: 4,649

  • Controls
    Cell Surface Library includes 500 non-targeting controls
  • Lentiviral Generation
    3rd

Library Shipping

These libraries are delivered as suspended DNA in a microcentrifuge tube on blue ice (#207471 will be delivered as a single tube, #1000000228 will be delivered as two tubes). The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼15 µL
  • Concentration
    25 ng/µL

Resource Information

Depositor Comments

The depositing lab recommends using MAGeCK algorithms (Link opens in a new window) for analysis.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FNLCR CRISPRa Cell Surface Library was a gift from Raj Chari (Addgene #207471)
    FNLCR CRISPRa Cell Surface and Soluble Libraries was a gift from Raj Chari (Addgene #1000000228)

  • For your References section:

    Uncovering receptor-ligand interactions using a high-avidity CRISPR activation screening platform. Yang L, Sheets TP, Feng Y, Yu G, Bajgain P, Hsu KS, So D, Seaman S, Lee J, Lin L, Evans CN, Guest MR, Chari R, St Croix B. Sci Adv. 2024 Feb 16;10(7):eadj2445. doi: 10.1126/sciadv.adj2445. PubMed 38354234
Related items
Additional resources