Wright Human Membrane Protein Activation Library
(Pooled Library #113345)
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Purpose
This lentiviral CRISPR activation library targets the promoters of 6,213 genes that encode at least one putative membrane-associated protein isoform. It can be used for large-scale screening for receptor identification, antibody cross-reactivity assessments, or characterization of extracellular interactions.
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Vector Backbone
pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W (Plasmid #112925) - does not express Cas9
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Depositing Labs
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Pooled Library | 113345 | gRNA pooled library† | 1 | $540 | Add to Cart |
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA (Addgene #113341) or any other plasmid(s) or cell lines expressing both dCas9-VP64 and MS2-p65-HSF1.
Library Details
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SpeciesHuman
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Genes targeted6,213
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gRNAs58,570
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Controls500 non-targeting
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
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Volume∼20 µL
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Concentration50 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human Membrane Protein Activation library was a gift from Gavin Wright (Addgene #113345) -
For your References section:
Pooled extracellular receptor-ligand interaction screening using CRISPR activation. Chong ZS, Ohnishi S, Yusa K, Wright GJ. Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3. PubMed 30477585 (Link opens in a new window)