AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR
(Plasmid
#207878)
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Purpose(Empty Backbone) AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207878 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV-Guide-it-1
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Backbone manufacturerTakara
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Modifications to backboneIts original CMV promoter for SaCas9 expression was replaced by the EF1a core promoter. Two sgRNA cloning sites (BbsI and PaqCI for cloning) were added, and the Sa Guide scaffolds were changed to the engineered variant to prevent possible early termination of gRNA expression.
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Vector typeMammalian Expression, AAV, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR was a gift from Yeh-Hsing Lao (Addgene plasmid # 207878 ; http://n2t.net/addgene:207878 ; RRID:Addgene_207878) -
For your References section:
Focused ultrasound-mediated brain genome editing. Lao YH, Ji R, Zhou JK, Snow KJ, Kwon N, Saville E, He S, Chauhan S, Chi CW, Datta MS, Zhang H, Quek CH, Cai SS, Li M, Gaitan Y, Bechtel L, Wu SY, Lutz CM, Tomer R, Murray SA, Chavez A, Konofagou EE, Leong KW. Proc Natl Acad Sci U S A. 2023 Aug 22;120(34):e2302910120. doi: 10.1073/pnas.2302910120. Epub 2023 Aug 14. 10.1073/pnas.2302910120 PubMed 37579143
Map uploaded by the depositor.
